Abstract
Introduction
This study is the first to describe the genetic diversity of C. trachomatis strains derived from patients with signs and symptoms of genitourinary infections
admitted to Tehran health centers and hospitals using the high-resolution genotyping
method, multilocus variable-number tandem-repeat analysis with ompA sequencing (MLVA)-ompA.
Methods
One hundred and sixty-seven urogenital specimens were collected from October 2019
to July 2020. Specimens were inoculated to cell culture and examined for the presence
of C. trachomatis isolates by microscopic valuation. Out of 167 samples, 19 (11.3%) viable C. trachomatis organisms were isolated in cell culture. Eighteen isolates were successfully genotyped
by MLVA-ompA analysis.
Results
The most prevalent ompA genotypes were E, D, F and G, comprising 42%, 26.3% and 21% and 10.5% of isolates,
respectively. Other genotypes were not detected from any of the samples. Out of the
18 fully genotyped isolates, 10 different MLVA-ompA genotypes were obtained. The most prevalent MLVA-ompA genotypes were 8.6.1-E (33.3%) and 8.5.2-D (16.6%). Genotype 8.6.1-E was common in
both females and males.
Conclusions
Our results showed that MLVA-ompA analysis was more discriminatory than ompA typing alone and, therefore, a suitable complement to ompA. Using this method, dominant genotypes in the community and transmission patterns
in sexual networks could be identified. The high diversity of C. trachomatis strains in Tehran may be due to the low level of public health and awareness, and
future studies are needed.
Keywords
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Article info
Publication history
Published online: April 16, 2023
Accepted:
April 13,
2023
Received in revised form:
March 19,
2023
Received:
September 14,
2022
Publication stage
In Press Corrected ProofIdentification
Copyright
© 2023 Japanese Society of Chemotherapy and The Japanese Association for Infectious Diseases. Published by Elsevier Ltd. All rights reserved.
